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PURPOSE: This study was conducted to evaluate the effects of yoga on pain levels and functional disabilities in nurses. DESIGN: Randomized controlled trial. METHODS: The sample of the study consisted of 91 nurses (Intervention group: 44, control group: 47). Ethics committee and institutional approvals were obtained for the study to be conducted. Data of the study were collected using a questionnaire form, Mcgill Melzack Pain Questionnaire (MASF), Oswestry Functional Disability Scale. Descriptive statistics, t-test in independent groups, Paired t-test and chi-square tests were used in the evaluation of the data. RESULTS: A statistically significant decrease was found in all sub-dimensions of the MASF scale after the application of the nurses in the intervention group compared to before the application (p < .001). A significant decrease was found in the post-application mean scores of the Oswestry Functional Disability Scale of the nurses in the intervention group (p < .001). CONCLUSIONS: Four weeks of regular yoga practice reduced pain and functional disability in nurses CLINICAL IMPLICATIONS: Yoga-based interventions may help nurses maintain physical health and achieve successful pain management.