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To the Editor: The mRNA-1273 vaccine against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) elicited high neutralizing-antibody titers in phase 1 trial participants1,2 and has been shown to be highly efficacious in preventing symptomatic Covid-19 disease and severe disease.3 The emergence of SARS-CoV-2 variants in the United Kingdom (the B.1.1.7 lineage), South Africa (the B.1.351 lineage), Brazil (the P.1 lineage), and California (the B.1.427/B.1.429 lineage) has led to concerns about increased transmission and the potential of these variants to circumvent immunity elicited by natural infection or vaccination. The recent identification in the United Kingdom of a B.1.1.7 variant that includes the E484K mutation (B.1.1.7+E484K) furthers these concerns. We assayed the neutralizing activity against recombinant vesicular stomatitis virus (rVSV)–based SARS-CoV-2 (a pseudovirus-based model) in serum samples obtained from eight participants in the phase 1 trial. The samples were obtained 1 week after the participants had received the second dose of mRNA-1273 vaccine. We tested pseudoviruses bearing the spike proteins from the original Wuhan-Hu-1 isolate, the D614G variant, and the B.1.1.7, B.1.351, P.1, B.1.427/B.1.429, B.1.1.7+E484K, and other variants (20E [EU1], 20A.EU2, N439K-D614G, and the mink cluster 5 variant that was first identified in Denmark).

dose of mRNA-1273 vaccine. We tested pseudoviruses bearing the spike proteins from the original Wuhan-Hu-1 isolate, the D614G variant, and the B.1.1.7, B.1.351, P.1, B.1.427/B.1.429, B.1.1.7+E484K, and other variants (20E [EU1], 20A.EU2, N439K-D614G, and the mink cluster 5 variant that was first identified in Denmark). Both the full panel of mutations in S and a subset of mutations affecting the receptor-binding domain (RBD) region of the B.1.1.7 variant had no significant effect on neutralization by serum obtained from participants who had received the mRNA-1273 vaccine in the phase 1 trial (Figure 1A and 1B). In contrast, we observed a decrease in titers of neutralizing antibodies against the P.1 variant, the B.1.427/B.1.429 variant (versions 1 and 2), the B.1.1.7+E484K variant, and the B.1.351 variant as well as a subset of its mutations in the RBD. We detected reductions by a factor of between 2.3 and 6.4 in titers of neutralizing antibodies against this panel of variants (Figure 1C through 1I). The largest effect on neutralization, reduction by a factor of 6.4, was measured against the B.1.351 variant (Figure 1C and 1D). However, the geometric mean neutralizing titer against B.1.351 was 1:290, and all the serum samples fully neutralized the rVSV pseudovirus, albeit at relatively low dilutions (Fig. S1 in the Supplementary Appendix, available with the full text of this letter at NEJM.org). The effect of the E484K mutation was observed by comparing neutralizing activity against the B.1.1.7 variant with neutralizing activity against the B.1.1.7+E484K variant. We found a significant reduction in neutralizing titers when the E484K mutation was present (Figure 1B and 1I). Using both rVSV and lentiviral neutralization assays, we observed a similar trend in serum samples obtained from macaque monkeys (Figs. S2 and S3).

ith neutralizing activity against the B.1.1.7+E484K variant. We found a significant reduction in neutralizing titers when the E484K mutation was present (Figure 1B and 1I). Using both rVSV and lentiviral neutralization assays, we observed a similar trend in serum samples obtained from macaque monkeys (Figs. S2 and S3). The rVSV-based pseudovirus neutralization assay was also used to assess the neutralizing activity of serum obtained from participants who had received the mRNA-1273 vaccine in the phase 1 trial against the full-length spike protein of the dominant strain in 2020 (D614G), as well as against 20E (EU1), 20A.EU2, N439K-D614G, and mink cluster 5 variants (Table S1). We observed levels of neutralization against these variants that were similar to those against the Wuhan-Hu-1 (D614) isolate (Fig. S4). Protection conferred by the mRNA-1273 vaccine against the P.1, B.1.427/B.1.429, and B.1.351 variants remains to be determined. Our findings underscore the importance of continued viral surveillance and evaluation of vaccine efficacy against new variants and may help to facilitate the establishment of correlates of protection in both nonhuman primates and humans.