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©2013 UpToDate ® Print Email Seventy-two hour fecal fat collection and calculation of coefficient of fat absorption 1. Forty-eight hours prior to the beginning of stool collection, subject is started on a high fat diet (>2 gm/kg/day and minimum of 50 gm/day fat). 2. All stools are collected into a single container for a full 72 hours. Stool container is kept refrigerated during collection. 3. During the collection, all food and fluid intake is recorded. A record of all bowel movements is kept. Intake record is analyzed for total dietary fat intake. 4. Collected stool is homogenized and analyzed for fat by the method of van de Kamer*. Medium chain triglyceride will not be measured by this method. 5. Coefficient of fat absorption (CFA) is calculated from the following equation: CFA = Dietary fat - Stool fat ------------------------ Dietary fat This protocol is intended for clinical use. This protocol is not sufficiently precise to be used for research purposes. * Van de Kamer, JH, et al. Rapid method for the determination of fat in feces. J Biol Chem 1949; 177:347. Courtesy of Robert D Baker, MD.