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©2013 UpToDate ® Print Email Acute fatty liver of pregnancy High power view of an oil red O stain of a liver biopsy from a patient with acute fatty liver of pregnancy. There are vacuolated hepatocytes containing microvesicular fat which stain red (arrows). This stain should be routinely used for the diagnosis of acute fatty liver of pregnancy and must be performed on a biopsy specimen that has not been fixed. Courtesy of Caroline A Riely, MD.