Browse the corpus
Walk the Even Hospital Database by book and chapter — the raw source passages that ground Ask, DDx, and the rest.
1 passage
©2013 UpToDate ® Print Email Detection of common alpha thalassemias by gap PCR Detection of the Southeast Asian (SEA) deletion by gap PCR. Lanes 1 and 8: DNA size markers (1 kb ladder). Each patient sample is run in duplicate, with the first lane (lanes 2, 4, and 6) using primers that bridge the normal sequence and the second lane (lanes 3, 5, and 7) using primers that bridge the SEA deletion. Lanes 2 and 3 and lanes 6 and 7: heterozygous SEA deletion. Lanes 4 and 5: normal control. Note that the SEA deletion (660 bp fragment) and the normal sequence (980 bp) are both seen in the subject with heterozygous SEA deletion, whereas only the 980 bp fragment is seen in the normal subject. PCR: polymerase chain reaction.