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Walk the Even Hospital Database by book and chapter — the raw source passages that ground Ask, DDx, and the rest.

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contentuptodate· Content· item f34_9_34967

©2013 UpToDate ® Print Email Beta chain variants reverse HPLC Reversed phase HPLC separation of variant beta-globin chains. Normal beta and alpha chains are highlighted. The separation of hemoglobins into their component globin chains and heme, which is eluted earlier, is achieved by the chemical composition of the buffer used in this technique, namely 0.1 M trifluoroacetic acid (TFA) and acetonitrile. HPLC: high performance liquid chromatography.