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©2013 UpToDate ® Print Email Direct immunofluorescence of bullous pemphigoid Direct immunofluorescence discloses linear deposits of IgG and C3 along the dermoepidermal junction. Ultrastructurally, these antibodies and complement are present in the lamina lucida. Reproduced with permission from: Rubin, E, Farber, JL. Pathology, 3rd ed, Lippincott Williams & Wilkins, Philadelphia 1999. Copyright ©1999 Lippincott Williams & Wilkins.