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©2013 UpToDate ® Print Email Linear C3 basement membrane zone antibody staining in bullous pemphigoid by direct immunofluorescence microscopy Direct immunofluorescence microscopy of perilesional skin from a patient with bullous pemphigoid reveals characteristic linear C3 basement membrane zone staining. Courtesy of Kristin M Leiferman, MD, Immunodermatology Laboratory, Department of Dermatology, University of Utah, Salt Lake City, Utah.