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©2013 UpToDate ® Print Email Capillary electrophoresis of hemoglobin variants Separation pattern and quantification of an artificial mixture of normal and some common variant hemoglobins by capillary electrophoresis (courtesy of Sebia®). The first of the two boxed items (top) is the actual capillary electrophoretic separation pattern of the hemoglobins seen in the graph. For quantification, these are scanned at 415 nm wavelength and the relative percentage is calculated, as shown in the second (bottom) box.